Anti-EGFR Monoclonal Antibody
應(yīng)用 WB IF IHC
稀釋比例WB 1:500-2000. IF 1:100-200. IHC 1:200-500.
交叉反應(yīng)Human

產(chǎn)品型號：K200050M

更新時間：2022-08-15

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抗體

標(biāo)簽抗體血清球蛋白 IgG Polyclonal Antibody Antibody Monoclonal Antibody 一抗內(nèi)參抗體膠體金標(biāo)記二抗生物素化二抗 RBITC熒光標(biāo)記抗體二抗 FITC熒光標(biāo)記抗體二抗免疫血清二抗純化抗體二抗 HRP標(biāo)記二抗免疫球蛋白抗原IgG二抗

FITC熒光標(biāo)記抗體該如何進(jìn)行標(biāo)記,有哪些標(biāo)記方法?

產(chǎn)品介紹

Anti-EGFR Monoclonal Antibody

英文名稱	Anti-EGFR Monoclonal Antibody
宿主	Mouse
別名	Epidermal growth factor receptor;erbb 1;Receptor tyrosine protein kinase ErbB 1
應(yīng)用	WB IF IHC
稀釋比例	WB 1:500-2000. IF 1:100-200. IHC 1:200-500.
交叉反應(yīng)	Human
蛋白分子量	170kDa
Gene ID	1956
保存	Store at -20°C. Avoid freeze / thaw cycles.
儲存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
純化方法	Affinity purification
亞型	IgG1
免疫原	A synthetic peptide coupled to KLH
性狀	液體
Public Immunogen Range	A synthetic peptide coupled to KLH
Subcellular Locations	Nucleus Endosome Plasma membrane Golgi apparatus Endoplasmic reticulum secreted
Swiss Prot	P00533
克隆號	1B1
克隆類型	Monoclonal Antibody
背景資料	The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.