sciencell  內皮細胞培養基（ECM）

Endothelial Cell Medium(ECM)

Catalog Number: 1001

Product Description

Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human  microvascular endothelial  cells  in vitro.  It is a sterile, liquid medium  which  contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in  an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of  endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of  penicillin/streptomycin solution (P/S, Cat. No. 0503). 

Product Use

ECM is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Store the basal medium at 4℃, the ECGS, the FBS and the P/S solution at -20℃. Protect from light. 

Shipping

Gel ice.

Prepare for use

Thaw  ECGS, FBS and P/S solution at 37℃.  Gently tilt the  ECGS tube several times during thawing  to help the contents dissolve.  Make sure the contents of the supplement are compley dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ECGS, FBS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37℃. When stored in the dark at 4℃, the reconstituted medium is stable for one month.

Caution:

If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

美國ScienCell研究實驗室成立于1999年，公司總部位于美國加州的圣地亞哥。主要致力于實驗室科研用原代細胞、原代細胞培養基、原代細胞無血清培養基、干細胞、干細胞培養基、干細胞無血清培養基的研究和開發，在擁有眾多客戶。在國內銷售8年來，很多老師應用其產品發表了高質量的SCI文章，且有著的文獻引用率。憑借著嚴格的質控和的產品品質，深受廣大科研工作者的信賴。
       ScienCell研究實驗室生產的原代細胞、原代細胞培養基都經過了嚴格的質量控制，細胞純度可達98％。其中包括21種人體正常細胞系統，90多種不同細胞類型。大多數細胞在唯有ScienCell實驗室能夠成功分離，產品質量過硬。確保了實驗結果的重復性和連貫性。

內皮細胞培養基（ECM）是專門為正常人類微血管內皮細胞體外培養設計的*適于其生長的*培養基。內皮細胞培養基（ECM）是經滅菌的液體培養基，包含必需和非必需氨基酸、維生素、有機和無機化合物、***、生長因子、微量礦物質和低濃度胎牛血清(5%)。內皮細胞培養基（ECM）緩沖體系為重碳酸鹽，在含5%CO2的細胞培養箱中平衡后pH值為7.4。內皮細胞培養基（ECM）的配方能夠選擇性的促進正常人類微血管內皮細胞體外培養中的增殖和生長，并為其達到*理想營養平衡狀態提供數量上和質量上的保證。
      內皮細胞培養基（ECM）包含500ml基礎培養基，25ml胎牛血清(FBS,目錄編號0025)，5ml內皮細胞生長添加物(ECGS,目錄編號1052)，和5 ml青霉素/鏈霉素溶液(P/S,目錄編號0503)

sciencell  內皮細胞培養基（ECM）訂購說明：

1、絕大部分產品備有現貨，一般情況下都能訂貨當日發貨。 
2、部分非常用產品，需提前1－2日預訂，海外期貨則需要提前3-6周預訂。 
3、部分產品價格會因貨期、批次等因素發生變化，若有變動以訂貨當日新價格為準。 
4、每日訂單截止時間為16點整，部分城市可到17點整，因超過截止時間造成當日不能發貨的將于次日安排發貨。
5、請辦理完貨款后，將收據、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。

sciencell  內皮細胞培養基（ECM）運輸說明：

極低溫產品：極低溫產品運輸過程中加裝干冰運輸。用干冰把產品包裹起來，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產品的性質穩定如一，為您的實驗保駕護航。
低溫產品：低溫產品運輸過程中加裝索萊寶冰袋運輸。事先用冰袋把產品包裹起來，再使用泡沫盒密封，用膠帶嚴實密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續一周），然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產品的性質穩定如一，為您的實驗保駕護航。
常溫產品：常溫產品運輸過程中無需加冰或者特殊包裝。產品由公司庫房人員快速配貨，準確快速高效的快遞保證產品快速送達您的手中。